Villa will begin the new Barclays U21 Premier League campaign with a home fixture against Stoke.
Gordon Cowans' team are set to welcome the Potters in their first game of 2014-15, which will take place at Bodymoor Heath.
The claret and blues will compete in Division Two of the revamped competition, having finished 15th in last season's 22-team league.
The 12 teams in Division Two will play each other home and away, with Villa's home fixtures split between Villa Park, the Pirelli Stadium in Burton and the club's Bodymoor Heath training ground.
There will be four opportunities for supporters to see the Young Lions at Villa Park over the course of the campaign, starting with Arsenal on Thursday September 11.
West Bromwich Albion then visit B6 on Monday March 16, followed by Newcastle on Monday April 20 and Brighton on Monday May 18.
Villa's young guns will also get the chance to run out at The Hawthorns when they face the Baggies on Thursday October 16, St James' Park for the corresponding Newcastle fixture on Monday November 3, Brighton's Amex Stadium on Monday January 5, Reading's Madejski Stadium on Friday May 1 and the Riverside for the Middlesbrough clash on Friday May 8.
Meanwhile, Villa U21s will travel to Swindon Town for an additional pre-season friendly this Saturday.
The clash at the County Ground will kick off at 3pm.
2014-15 U21 league fixtures:
Mon Aug 18 - H - Stoke City - 1pm - Bodymoor Heath
Mon Aug 25 - A - Blackburn - 7pm - Leigh Sports Village
Thur Sep 11 - H - Arsenal - 7pm - Villa Park
Mon Sep 22 - A - Derby - 1pm - Derby County Academy
Mon Sep 29 - H - Bolton - 1pm - Bodymoor Heath
Thur Oct 16 - A - West Brom - 7pm - The Hawthorns
Tue Oct 28 - H - Wolves - 7pm - Pirelli Stadium
Mon Nov 3 - A - Newcastle - 7pm - St James' Park
Mon Dec 1 - H - Reading - 7pm - Pirelli Stadium
Mon Dec 15 - H - Middlesbrough - 7pm - Pirelli Stadium
Mon Jan 5 - A - Brighton - 7pm - Amex Stadium
Fri Jan 9 - A - Stoke - 7pm - Moss Rose Ground
Mon Jan 26 - H - Blackburn - 7pm - Pirelli Stadium
Wed Feb 11 - H - Derby - 7pm - Pirelli Stadium
Mon Feb 23 - A - Arsenal - 7pm - Boreham Wood
Mon Mar 9 - A - Bolton - 7pm - Macron Stadium
Mon Mar 16 - H - West Brom - 7pm - Villa Park
Mon Apr 6 - A - Wolves - 7pm - AFC Telford
Mon Apr 20 - H - Newcastle - 7pm - Villa Park
Fri May 1 - A - Reading - 7pm - Madejski Stadium
Fri May 8 - A - Middlesbrough - 7pm - Riverside Stadium
Mon May 18 - H - Brighton - 7pm - Villa Park