Villa fielded one of their youngest-ever sides at this level in the defeat to Arsenal.
Jerell Sellars kept up his impressive scoring form with two goals for the claret and blues, who gave debuts to U15 players Easah Suliman, Corey Taylor and Andre Green.
But the Gunners rallied with goals from Alfred Mugabo, Jack Jebb, Anthony Jeffery, Austin Lipman and Alex Iwobi.
VILLA: Watkins, Suliman, Wildin, Zazrivec, Lewis, Toner, Cowans, Sellars, T Strain, Calder, Abdo. Subs: Lyden, Grant, Taylor [for Abdo, 55], Green [for Calder, 60], R Strain [for Zazrivec, 73].