Ron Vlaar, Andreas Weimann, Nathan Baker, Gabby Agbonlahor and Charles N'Zogbia are all injury doubts ahead of Wigan.
Villa's quality quartet are all struggling for Saturday's clash.
Vlaar has missed six games with a calf problem.
Weimann also injured his calf in the game at Chelsea and was absent from the Tottenham match.
Baker limped off against Tottenham with a hamstring problem while Agbonlahor and N'Zogbia have hip and knee issues respectively.
Lambert said: "The injury situation is not helping us at the minute. There are a lot at the football club. You have just got to go with what you have got though.
"With Ron, I don't know. He's doing alright at the minute but has not played since Arsenal.
"He has not trained with us. He's still working on his rehab.
"Nathan has a hamstring problem. We don't know how bad it is. Andreas has injured his calf.
"Gabby still has a problem with his hip area and with Charles it's his knee. They are still struggling a bit."
Asked if any might come back for Wigan, he added: "I am not so sure. I don't think there will be many of them coming back."
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