Gabby Agbonlahor will see a specialist regarding his knee injury when Villa arrive back from their tour of the USA.
Agbonlahor underwent a scan in Portland on Monday after sustaining the injury in Saturday's 1-0 win over Chicago Fire.
The Villa striker scored the only goal of the game at Toyota Park but was forced off after injuring his knee in the second period.
No timeframe has been determined for Agbonlahor's absence, although his recovery is likely to take longer than first anticipated.
The 25-year-old was forced to miss out on Villa's final USA tour match against Portland Timbers on Tuesday.
The boys in claret and blue rounded off their trio of Stateside fixtures with a penalty shootout success after drawing 2-2 in normal time.
It means Paul Lambert's men return home with an unbeaten record having earned 1-0 wins over Philadelphia Union and Chicago Fire.
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