James Collins, Gabby Agbonlahor and Carlos Cuellar all came through 45 minutes of a behind closed doors game at Bodymoor Heath.
Agbonlahor was among the goalscorers as Villa ran out comfortable 5-0 winners over Burton Albion.
"Depending on how their bodies react, all three players should be fit and in the squad for the second leg of our Europa League play-off game against Rapid Vienna on Thursday," said caretaker boss Kevin MacDonald.
"We will have to wait and see if there are any reactions but at this moment we are hopeful that they will be able to return."
Harry Forrester helped himself to a brace in the victory, while fellow strikers James Collins Jr and Nathan Delfouneso were also on target.